Abstract
Orange fruit rind extract has a stimulative effect on the spore germination of Penicillium digitatum (Green mold of citrus fruit). The stimulative factor was readily extracted from the rind by water and absorbed by activated charcoal (active carbon).
The component of the extract from the rind absorbed by charcoal was released by 60-80% acetone. The concentrated material from acetone extract had the stimulative effect on the spore germination of P. digitatum only when added with the filtrate (charcoal treated extract) or with very small amount of the phosphates. Thus, the phosphoric compounds were found to be the chemicals to activate the germination stimulant.
It is suggested that the factor to stimulate the spore germination of P. digitatum seems to consist of two fractions, namely the one which is absorbed by charcoal and the other is not, or that the factor would be separated in two components by charcoal treatment and would recover by adding the phosphate compounds.