Abstract
In the previous reports, abnormal phosphorous metabolisms were observed in both the dwarf virus-infected mandarin plant and the virus transmitting-green broad winged planthopper. This paper deals with the experiments to elucidate the rate of incorporation of P32 into the phosphorous fractions in these abnormal metabolisms.
(1) The radioactivity of P32 in various phosphorous fractions of mandarin plant or planthopper shows relatively higher level, as affected with the virus (Tables 1∼2).
(2) The acid soluble phosphorous compounds of the examined material were fractionated by the method of LePage and Umbreit. In both the plant and the insect, the amounts of phosphorous contents in the various fractions show no consistent difference in trend between healthy and diseased individuals. The radioactivity in each fraction of the viruliferous insect is, however, found on considerably higher level than that of the nonviruliferous one (Tables 3-4 and Figs. 1-2).
(3) The nucleic acids examined were extracted by the method of Ogur-Rosen and Schneider from orange plant, and by the Schneider's method from planthopper. The incorporation of P32 into the nucleic acids is considerably lower rate, and the autoradiograms present no difference between healthy and diseased plant or between nonviruliferous and viruliferous planthopper, respectively (Figs. 3∼4). It is suggested that the nucleic acid is to be prepared a more suitable technique under milder conditions.
