Abstract
1) Using Ouchterlony's agar-gel diffusion technique, it was shown that leaf juice of diseased barley, wheat, or sweet corn infected with barley stripe mosaic virus (BSMV) contains an antigenic substance which react specifically against anti-BSMV sera.
2) The resulting precipitation appeared in the agar layer as a distinct sickle-shaped band near the hollow filled with the diseased leaf juice sample. The antigenic substance corresponding to this band was considered to be the virus.
3) Antisera prepared with immunogen purified by salting-out with ammonium sulfate at two-fifths saturation contained, in addition to the antibody against the virus-antigen, several antibodies which react against healthy plant components.
4) Antisera prepared with immunogen purified by differential centrifugation usually did not contain any detectable amount of antibodies against healthy plant components.
5) The source of all antibodies except that against the virus-antigen was considered to be normal plant proteins.
