Further studies on the rice dwarf virus.-II
1962 Volume 27 Issue 4 Pages 204-213
Details
1962 Volume 27 Issue 4 Pages 204-213
1. When extracts from leaves of diseased rice plants or from viruliferous leafhoppers were centrifuged at 10, 000rpm for 30 minutes the virus was slightly precipitated, but it was completely precipitated by centrifugation at 20, 000rpm for 120 minutes. Partially purified preparations of the virus were secured by two cycles of alternate low and high speed centrifugations. The sedimentation constant of the virus particle in M/30 phosphate buffer was determined to be 405×10-13sec.
2. Nucleic acid extracted from partially purified virus preparations with water saturated phenol and sedimented with NaCl was found to be infective, when injected into the abdomen of non-viruliferous leafhoppers, but the preparations sedimented with ethanol were devoid of infectivity.
3. When rabbits were injected intramuscularly with an emulsion of partially purified antigen in Freund's adjuvant, a high titer antiserum was obtained.
4. The virus could not be cultivated on the chorioallantoic membrane of developing chick embryo.
