Abstract
An unidentified ninhydrinpositive substance (NPS) was detected in the culture filtrate of several plant pathogenic fungi by thin layer chromatography when cultured on the modified Czapek's medium containing authentic amino acid (s). The chemical property was different from that of alanine, glycine, threonine, glutamic acid, glutamine, glucosamine, galactosamine, and anthranilic acid in thin layer chromatographic behaviors. At an early stage of the mycelial growth, NPS appeared in each combination between amino acids used in the modified Czapek's medium and plant pathogenic fungi employed. Consequently, the authors concluded that plant pathogenic fungi have an ability through a common metabolic pathway to produce NPS in the culture medium.