Pycnidium Formation by Fifty Different Isolates of Macrophomina phaseoli Originated from Soil or Kidney Bean Seed

Abstract

Among 50 different isolates originated from field soil, kidney bean seed, or contaminated dirt of seed tested, 5, 11, or 16 respective isolates formed pycnidia on dried hypocotyl segments placed on 1.5% water agar under artificial light of 2, 000-3, 000lux at 30°C, natural light in shade at 26°C, or at fluctuating temperature of 18-42°C, respectively. Among them, isolates no.12, no.51, and no.179 were rather excellent in pycnidium formation. Environmental conditions such as temperature and light are influencial for pycnidium formation in addition to the inherent ability of pycnidium formation in the respective isolates.