1935 Volume 4 Issue 3-4 Pages 178-190
The present paper deals with the results of the author's experiments on the biological characters of pseudo-myceliolyse of Ophiobolus Miyabeanus ITO et KURIBAYASHI (=Helmlnthosporium Oryzae BREDA de HAAN) with special reference to the effect of temperature and constituent of media on the occurrence of the phenomenon, and the rate of enlargement, the time required of incipient and final stage of pseudo-myceliolyse.
The temperature has a profound effect on the occurrence of pseudomyceliolyse. The optimum temperature for pseudo-myceliolyse on potato sucrose agar lies at 23 to 28°C, especially at about 24°C.
No pseudo-myceliolyse occurred in cultures grown at 16°C, or temperature lower than this and at 37°C, or above than this.
Pseudo-myceliolyse occurred frequently on such various liquid or agar media as potato sucrose, rice straw decoction, synthetic media with asparagine, and peptone, corn meal and apricot decoction.
No peudo-myceliolyse occurred on MIYOSHI's and SAITO's onion soy ager.
Pseudo-myceliolyse increased its area at the rate of 0.496 to 5.106, average 3, 511mm spuare per minute at 23°C.
In a certain part of the area of pseudo-myceliolyse developed at the rate of 0.083 to 0.5, average 0.291μ per minute at 17 to 20°C.
The incipient stage of psedo-myceliolyse appeared in 54 to 74, average 64 hours at 32°C and 49 to 69, average 59 hours at 23°C after inoculation.
The final stage of pseudo-myceliolyse was 72 to 95 hours from cultures were started, and was 20 hours in average from its incipient stage.
The pseudo-myceliolyse could not, therefore, been recognized unless a detailed and continuous observation has been made.