Preparation of Mouse Ascitic Antibody against Tobacco Stunt Disease Antigen and its Application for Serodiagnosis

Abstract

Tobacco stunt disease (TSD) antigen was partially purified by differential centrifugation from infected tobacco leaves showing typical symptoms of TSD. The partial purified preparation was mixed with equal volume of Freund's complete adjuvant, and was injected three times to mice weekly followed by tumor injection. Ascitic fluids in immunized mice contained both TSD antibody and healthy tobacco antibody. The ascitic fluid absorbed with healthy tobacco antigen react with TSD antigen only. Mouse antibody against Hatano isolate of TSD formed a precipitin line between the antigen of Iwate isolate as same as Hatano isolate of TSD by agar diffusion test. It was shown that serodiagnosis of naturally infected TSD can be used by agar diffusion tests using absorbed mouse ascite.