Spray Inoculation with Conidial Suspension of Fusarium Leaf spot Fungus of Rice Plant

Abstract

Spray inoculation with water suspension of conidia of Fusarium leaf spot fungus (Fusarium nivale (Fries) Cesati) was unsucceessful. However, the conidial germination and fusion of their germ tubes by which stomatal penetration was conducted were enormously promoted by the addition of polypeptone to spore suspension. Inoculation was accomplished by spraying spore suspensions containing 1% polypeptone and the inoculated plants were incubated in a high humidity chamber at 25-28C for more than 36 hours.