Abstract
The previous studies made on the mode of action of sodium dimethyldithiocarbamate (NaDMDC) showed that the compound inhibited the lipid synthesis of Xanthomonas oryzae, but not the process from long-chain fatty acids to phospholipids in the biosynthetic pathway of lipids. Further examinations were made on the effects of NaDMDC on the process from acetate to fatty acid formation. NaDMDC inhibited neither the two kinds of enzymes involving in the formation of acetyl CoA from acetate, acetate kinase and phosphotransacetylase, nor the enzyme concerning in the synthesis of malonyl CoA from acetyl CoA, acetyl CoA carboxylase. However, the compound had a high inhibitory effect on the incorporation of 14C-malonyl CoA into fatty acids, in either the fatty acid-synthesizing enzyme system of Escherichia coli or the cellfree extracts of X. oryzae.
From these results, it is concluded that NaDMDC inhibits primarily the fatty acid synthesis of X. oryzae, and gives a remarkable influence upon the entire lipid biosynthesis.
