Axenic Culture of Two Rust Fungi, Puccinia coronata f. sp. avenae and P. recondita f. sp. tritici

Abstract

Urediniospores of Puccinia coronata f. sp. avenae race 367 and P. recondite f. sp. tritici race 45 were respectively cultured on an artificial medium. Urediniospores free of contaminating microorganisms were obtained by aseptic leaf culture. After the urediniospores were separately seeded on the medium, both rust fungi grew well and then formed white macroscopic colonies which had abundant aerial hyphae. About 1.5-2 months after inoculation part of the colonies changed from white to dark brown. Microscopic examination showed that spore-like bodies similar in morphology to urediniospores and teliospores were formed around the stroma. In the culture of P. coronata f. sp. avenae, teliospore-like bodies did not show a crown on top of the spores.