1980 Volume 46 Issue 5 Pages 647-655
The enzyme-linked immunosorbent assay (ELISA) provided a rapid and accurate method for detecting watermelon mosaic virus (WMV) and cucumber mosaic virus (CMV) in leaf extract and purified virus preparations from cucurbitaceous plants, pumpkin and cucumber. The reaction was specific enough to distinguish WMV and CMV even when the plants were infected doubly with the two viruses. ELISA was sensitive in detecting WMV and CMV in various parts, cotyledons, leaves, hypocotyls, and roots from plants 1 week after mechanical inoculation with crude extracts. The virus antigens in leaf disks dessicated with CaCl2 could be preserved and was easily detected with high ELISA absorbance values even at 30 C at least for 180 days' storage for WMV and over a storage period of 90 days for CMV. The possible application of the technique, together with dehydrated infected tissues for routine large scale diagnosis of WMV and CMV is discussed.