Japanese Journal of Phytopathology
Online ISSN : 1882-0484
Print ISSN : 0031-9473
ISSN-L : 0031-9473
The Detection of Watermelon Mosaic and Cucumber Mosaic Viruses in Cucurbitaceous Plants by Enzyme-linked Immunosorbent Assay
Nobumichi SAKOKazutoshi MATSUOFukuji NONAKA
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1980 Volume 46 Issue 5 Pages 647-655

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Abstract

The enzyme-linked immunosorbent assay (ELISA) provided a rapid and accurate method for detecting watermelon mosaic virus (WMV) and cucumber mosaic virus (CMV) in leaf extract and purified virus preparations from cucurbitaceous plants, pumpkin and cucumber. The reaction was specific enough to distinguish WMV and CMV even when the plants were infected doubly with the two viruses. ELISA was sensitive in detecting WMV and CMV in various parts, cotyledons, leaves, hypocotyls, and roots from plants 1 week after mechanical inoculation with crude extracts. The virus antigens in leaf disks dessicated with CaCl2 could be preserved and was easily detected with high ELISA absorbance values even at 30 C at least for 180 days' storage for WMV and over a storage period of 90 days for CMV. The possible application of the technique, together with dehydrated infected tissues for routine large scale diagnosis of WMV and CMV is discussed.

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