Serological Detection of Barley Yellow Mosaic Virus, Wheat Yellow Mosaic Virus and Soil-borne Wheat Mosaic Virus by ELISA

Abstract

Enzyme-linked immunosorbent assay (ELISA) was used to detect wheat yellow mosaic virus (WYMV), barley yellow mosaic virus (BYMV) and soil-borne wheat mosaic virus (SBWMV)in leaf and root extracts of infected barley and wheat. The γ-globulin and enzyme conjugated γ-globulin were used at 2μg/ml and 1:800 dilution, respectively. WYMV, BYMV and SBWMV were detected by ELISA in extracts of infected leaves diluted up to 25, 600, 6, 400 and 12, 800 times with phosphate buffered saline containning 0.05% Tween 20 (PBS-T), respectively. In the case of roots, WYMV and BYMV were detected in extracts with 0.1M, pH 7.0 citrate buffer, but not with PBS-T. In ELISA using antisera against WYMV and BYMV, both rice necrosis mosaic and oat mosaic viruses were not detected. ELISA was developed to index large number of plants of the breeding field in place of X-body or electron microscope observation.