Abstract
A crude self-inhibitor of Botrytis cinerea was successfully extracted from highly concentrated spore suspension by ethylacetate (EtOAc). The inhibitory spot was shown at Rf 0.5-0.6 by the following procedure: after development with EtOAc: cyclohexane=1:1 (v/v), the thin-layer chromatogram was covered with water agar, and cellophane membrane was laid on the solid water agar, and then spores were spread over cellophane membrane. The ED50 of the crude extract determined from a plot of the percent inhibition of germination against the logarithm of concentration was 642ppm. The inhibitory activity was not lost after heating for 10min at 121C. The substance was permeable through cellophane membrane.
