Zucchini Yellow Mosaic Virus Isolated from Pumpkin in Miyako and Yaeyama Islands, Okinawa, Japan

Abstract

Zucchini yellow mosaic virus (ZYMV) was isolated from Cucurbita maxima (cv. Ebisu) cultivated in Okinawa prefecture. The isolates were mechanically transmitted to 15 plant species belonging to 5 families, causing systemic infections in all cucurbitaceous species tested, and nonpersistently transmitted by Myzus persicae and Aphis gossypii. Filamentous particles (700-800nm long) were found in both leaf extracts and purified preparations. The isolates reacted with ZYMV antiserum from Italy in immunoelectron microscopy and SDS-immunodiffusion test.