1988 Volume 54 Issue 2 Pages 210-216
Pea stem necrosis virus (PSNV) was readily purified from infected pea plants by a chloroformbutanol procedure with yields of about 200mg/kg leaf tissues. Purified virus preparations contained a single sedimenting component with a sedimentation coefficient of 118S, buoyant density in cesium chloride of 1.35g/cm3 and nucleic acid content of 17%. Polyacrylamide gel electrophoresis demonstrated single nucleic acid component and single coat protein with estimated molecular weights of 1.53×106 and 38, 000 daltons, respectively. Nucleic acid extracted from the virus preparations was identified as single-stranded RNA by the following properties: positive orcinol reaction; susceptibility to RNase A both in 1×SSC or 0.1×SSC; increase of about 21% in maximum absorption and shift both the absorption maximum and minimum to higher wave-lengths by 4 nm on treatment with formaldehyde (1.8%). Preparations of purified particles of PSNV reacted with homologous antiserum (gel diffusion titer, 1:1, 024) but not with any antisera to 8 different plant viruses with isometric particles including tomato bushy stunt, galinsoga mosaic, carnation mottle or melon necrotic spot viruses. However, physicochemical properties were similar to those of carnation mottle and allied viruses.