Japanese Journal of Phytopathology
Online ISSN : 1882-0484
Print ISSN : 0031-9473
ISSN-L : 0031-9473
Detection of Resting Spores of Plasmodiophora brassicae Woron. from Soil and Root by Fluorescent-Antibody Technique
Tsutomu ARIEShigetou NAMBAShuichi YAMASHITAYoji DOI
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1988 Volume 54 Issue 2 Pages 242-245

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Abstract

Indirect fluorescent-antibody technique was applied for diagnosis of the clubroot of Brassicaceae caused by Plasmodiophora brassicae Woron. IgG (γ-globulin; 0.91 mg/ml) was purified from antiserum against resting spores of P. brassicae prepared from a rabbit. Infested soil and root were stained by fluorescent-antibody technique with the IgG and FITC conjugated antirabbit IgG-sheep IgG. (Cappel Lab.) Resting spores were effectively detected, and also clearly differentiated from small particles of soil and tissues of plant in the reflected light fluorescence microscope (Olympus BHS-RF-A, B-excitation). This method is considered to be suitable for the detection of P. brassicae.

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