1988 Volume 54 Issue 2 Pages 242-245
Indirect fluorescent-antibody technique was applied for diagnosis of the clubroot of Brassicaceae caused by Plasmodiophora brassicae Woron. IgG (γ-globulin; 0.91 mg/ml) was purified from antiserum against resting spores of P. brassicae prepared from a rabbit. Infested soil and root were stained by fluorescent-antibody technique with the IgG and FITC conjugated antirabbit IgG-sheep IgG. (Cappel Lab.) Resting spores were effectively detected, and also clearly differentiated from small particles of soil and tissues of plant in the reflected light fluorescence microscope (Olympus BHS-RF-A, B-excitation). This method is considered to be suitable for the detection of P. brassicae.