Abstract
To construct the restriction maps of the filamentous phage Xf and Xf2 DNA, the replicative form DNA (RF) was isolated from Xanthomonas campestris pv. oryzae cells infected with phage and digested with restriction endonucleases that recognize six bases. Xf-RF was cut by the treatment with restriction endonucleases BamHI, HindIII, SmaI and StuI at a respectively specific single site. Xf2-RF was cleaved by BamHI and XbaI at a single site, and PvuII and EcoRI at two sites. Based on the size and distribution patterns of the fragments generated by digestion with various combinations of these restriction endonucleases, restriction maps of the filamentous phage Xf-RF and Xf2-RF were constructed.
