Abstract
A selective medium was developed for isolation of Pseudocercosporella herpotrichoides from soil. This medium consists of potato dextrose agar supplemented with 0.3g streptomycin sulfate and 0.8-1.0g copper sulfate per liter. On this medium P. herpotrichoides forms a characteristic reddish-brown compact colony with thick mycelium 10-14 days after inoculation. Percent recovery of P. herpotrichoides from artificially infested soil was 18-54 or 16-60% on the medium containing 800 or 1, 000ppm copper sulfate, respectively. Naturally infested field soils yielded 3, 200-38, 000 propagules of P. herpotrichoides per gram of soil.
