Serological Detection of Four Plant Viruses in Cucurbitaceous Crops from Bangladesh

Abstract

In all 21 samples of various cucurbitaceous crops showing virus disease-like symptoms were collected in 1986-87 from different locations of Bangladesh and preserved at 4°C after lyophilization or with calcium chloride. These dried samples were used for serological detection of different cucurbit-viruses, viz., cucumber mosaic virus (CMV), papaya ringspot virus (PRSV/WMV 1), watermelon mosaic virus 2 (WMV 2), zucchini yellow mosaic virus (ZYMV), cucumber green mottle mosaic virus (CGMMV) and squash mosaic virus (SqMV) by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) and dot-immunobinding assay (DIBA) in 1989. Out of the 21 samples tested, two, nine, one and three were found to be positive against the antisera of CMV, PRSV, WWV 2 and SqMV, respectively. None of the samples reacted positively against the antisera of ZYMV and CGMMV. Some samples of bottlegourd, bittergourd, cucumber and pumpkin did not react with any of the antisera used.