Japanese Journal of Phytopathology
Online ISSN : 1882-0484
Print ISSN : 0031-9473
ISSN-L : 0031-9473
Bioassays for Induction of Dicarboximide Fungicide-Resistance with Filtrate of the Resistant Strains of Botrytis cinerea
Katsumi AKUTSUTomoyasu IKKATakeshi ENDOTakako OHARASatoshi OKUYAMA
Author information
JOURNALS FREE ACCESS

1992 Volume 58 Issue 4 Pages 534-537

Details
Abstract

Induction of dicarboximide fungicide-resistance by pre-culturing in the filtrates of the field resistant strains (IHES-2 and CAES-4) of Botrytis cinerea was examined by two bioassay methods. In the protoplast bioassay method, the regeneration frequencies of the mycelial protoplasts of the sensitive strain (IPCR-1) pre-treated with IHES-2 and CAES-4 filtrates, respectively, were 2.7 and 2.4% on the medium containing the fungicide, which were about tenfold higher than that by pre-treatment with IPCR-1 filtrate. In another method, IPCR-1 conidia were shaking-cultured in IHES-2 and CAES-4 filtrates for 3 days, respectively, and each of the conidia was harvested as a mycelial ball like structure (MBS). The MBSs pre-cultured in these filtrates formed the colonies about three times as many as those with IPCR-1 filtrate. From the two different bioassays, the induction of dicarboximide fungicide-resistance with the filtrates of the resistant strains was confirmed. Especially, MBS bioassay method may be a useful one for bioassays of filamentous fungi, in which it is hard to statistically detect the effects of physiologically active substances because of the morphogenic changes in the growth cycle.

Information related to the author
© The Phytopathological Society of Japan
Previous article Next article
feedback
Top