Japanese Journal of Phytopathology
Online ISSN : 1882-0484
Print ISSN : 0031-9473
ISSN-L : 0031-9473
Molecular Analysis of T-DNA Region on the Root Inducing Plasmid (Ri) in a Mikimopine Type Agrobacterium rhizogenes Strain 1724
Nobukazu TANAKATakeshi MATSUMOTOAtsuhiro OKA
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1993 Volume 59 Issue 2 Pages 155-162

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Abstract
The T-DNA region of the root-inducing (Ri) plasmid (pRi1724) in the mikimopine type Agrobacterium rhizogenes strain MAFF03-01724 isolated in Japan was detected and characterized by means of Southern blot hybridization with the T-DNA probe of the plasmid pRiA4b in the agropine type A. rhizogenes strain A4. Although the homologous sequences with TL-DNA of pRiA4b was found on pRi1724, no homologous sequence with TR-DNA was found. The presence of pRi1724 sequences was demonstrated in the genome of Ajuga reptans hairy roots by the infection with strain1724. The T-DNA of pRi1724 was mapped by means of cloned BamHI partial digests. The homologous sequences with virulence region of pRiA4b was also found.
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© The Phytopathological Society of Japan
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