Abstract
Axenic cultures of Puccinia recondita f. sp. tritici were established by placing aseptic leaf segments with spermogonia on artificial media. Good growth of colonies was observed on the medium which contained 25% concentration of Murashige and Skoog's minerals, vitamins, and glycine; sucrose, 40g; 2, 4-D, 0.001g; kinetin, 0.0001g; peptic peptone, 4g and bacto agar, 8g per liter. The colonies of the axenic cultures were white to yellow and felty to fluffy in appearance and consisted of narrow and straight hyphae. Microscopic observation and determination of the DNA content of the nucleus in hyphal cells showed that the cultures were haploid and uninucleate.
