Restriction Fragment Length Polymorphisms of Phytopathogenic Pseudomonads Probed with Biotinylated Escherichia coli rRNA

Abstract

Restriction fragment length polymorphisms (RFLP) of phytopathogenic pseudomonads were analyzed by Southern hybridization using biotinylated Escherichia coli 16S+23S rRNA as a probe. HindIII digests of total DNAs from 58 isolates including 11 species and 13 pathovars of pseudomonads showed extensive diversity and specificity of the rRNA gene RFLP patterns for the individual species and pathovars. These results suggest that this nonradioactive, simple and rapid method can be used for the practical discrimination of phytopathogenic pseudomonads.