Abstract
Total RNAs obtained from 26 isolates of 6 anastomosis groups (AGs) of Rhizoctonia solani were analyzed by Northern hybridization using the cloned fragment of the plasmid DNAs as the probes. Plasmid-encoded transcripts were found in all the plasmid containing isolates of R. solani. The sizes of the transcripts were distributed among 6 AGs of R. solani as follows: 6.8kb in AG1 IB, 2.8kb and 1.2kb in AG2-2 IIIB, 7.6kb and 2.7kb in AG2-2 IV, 5.8kb in AG3, 0.5kb in AG4, 3.6kb in AG5, and 8.0kb in AG6. Considerable sequence homology was observed among plasmid-encoded transcripts obtained from isolates of the same AG, but no sequence homology was found among transcripts obtained from isolates of different AGs and from isolates within the same AG which contain no plasmid.
