Detection of Japanese Yam Mosaic Virus by ELISA and RT-PCR

Abstract

Japanese yam mosaic virus was detected using ELISA and RT-PCR assays. In ELISA, Japanese (Dioscorea japonica) and Chinese yams (D. opposita) infected with JYMV reacted positively, but white yam (D. alata) infected with JYMV, Chinese yam infected with CYNMV and virus-free yam did not react. ELISA values differed among JYMV isolates. In RT-PCR, the PCR product was detected from all yams infected with JYMV, but not from yam infected with CYNMV and virus-free yam. These results suggest serological diversity among JYMV isolates.