2000 Volume 66 Issue 1 Pages 44-48
The pathogenicity and serological properties of Bean yellow mosaic virus (BYMV) isolates from both cultivated and naturalized gladioli in Kagoshima prefecture were investigated. According to the method of Sasaya et al. (1998), 64 BYMV isolates from gladiolus were classified as pathotype I or IV, whereas five BYMV isolates from broad bean were classified as pathotype II. The serological relationships among the BYMV isolates were determined with DAS-ELISA and the improved Ouchterlony double-diffusion test using antisera to isolates S-22N (pathotype I) and S-22C (pathotype IV) originating from a gladiolus source, and Sb-50C (pathotype II) from broad bean. The gladiolus isolates (S-22N and S-22C) differed serologically from the broad bean isolate (Sb-50C).