Abstract
In February 1999, cineraria (Senecio×hybridus) plants with necrotic ring spot were found in Okayama Prefecture, Japan. A spherical virus with a diameter of ca. 85∼120nm was isolated. The virus infected seven plant species of four families after mechanical inoculation. In serological tests, the virus was closely related to Impatiens necrotic spot virus (INSV). In RT-PCR with INSV-specific primers, a fragment of about 900bp was amplified. The fragment had a high degree of nucleotide sequence homology with the S RNA of INSV. Based on these results, the virus isolated from cineraria was identified as INSV. This is the first report of INSV on cineraria in Japan.
