Abstract
Coat protein (CP) of Apple stem grooving virus (ASGV) was obtained from E. coli expressing ASGV-CP gene. Purified CP was injected into a rabbit and antiserum to ASGV-CP was raised. The antiserum reacted with ASGV-CP in both western blots and double antibody sandwich-enzyme linked immunosorbent assays (DAS-ELISA). For DAS-ELISA on ASGV in citrus leaves, extraction buffers with different pH were tested. ELISA values were higher when extraction buffers of higher pH were used. Carbonate-bicarbonate buffer (pH 9.6) containing 0.05% Tween 20 gave the highest reading. This ELISA system was shown to be useful for detection ASGV in our surveys.
