2001 Volume 12 Issue 2 Pages 74-78
To elucidate whether low-power laser irradiation stimulates activation of dental pulp, effects of low-power laser (Ga-Al-As) irradiation on cellular growth and alkaline phosphatase (ALPase) activity were examined, using five populations of human dental pulp-derived fibroblast-like cells (HDP 1-5).
HDP were seeded into 96-well microplates and incubated in 10% FBS-containing a-MEM until confluency, for 3 days. After 24 h of serum starvation, a low-power laser (80 mW) was irradiated for 20 s, 1min, or 3min, once on day 5 (1 IR), or twice on days 5 and 6 (2 IR), respectively.
Cellular growth of HDP 1, 4, and 5 was accelerated to 120% compared with controls, by 1 min or 3 min of laser irradiation. ALPase activity of HDP 1, 2, 4, and 5 was stimulated up to 120-170% compared with controls, by 1min or 3min of irradiation. The above HDP responses induced by laser irradiation did not depend on irradiation frequency.
These results indicate that HDP responded to low-power laser irradiation, and the responses were effective on cellular differentiation rather than cellular growth.
