Original
Analysis of a factor released from UV light-irradiated Dictyostelium discoideum
2004 Volume 37 Issue 2 Pages 127-132
Details
2004 Volume 37 Issue 2 Pages 127-132
The behavior of the social amoeba Dictyostelium discoideum following UV irradiation was analyzed. Cells cultured in a liquid medium migrated at 4.5 ± 0.7 μm/min, while their motility increased to 19.4 ± 1.7 μm/min following UV irradiation (50 J/m2). A conditioned medium prepared from the irradiated cells, CM (UV), was used in the following experiments. Motility of cells that had not been exposed to UV increased to 29.7 ± 2.8 μm/min when the medium was changed to CM (UV). In addition, the expression levels of recA and rad51 were increased and sensitivity to UV was decreased in these cells. These results indicate that UV light-irradiated D. discoideum cells release a factor that enhances motility and induces expression of DNA repair enzymes in unirradiated cells. The factor would be important for D. discoideum to alleviate damage.
