Abstract
Localization of angiotensin-converting enzyme (ACE) and its activity in sarcoid granuloma were studied by ACE substrate film prepared from hippuryl-L-histidyl-L-leucine and purified calfskin gelatin. ACE activity in sarcoid granulomas was recognized as numerous sharply defined plaques (digestive vaculoes) or poorly stained plaques on the substrate film under epithelioid cells and the sites of sinus histiocytosis. ACE activity was completely inhibited by 10-1M of captopryl (SQ 14-225) or by 5×10-2M of EDTA.
Our substrate film method enables localization of ACE and the estimation of ACE activity in sarcoid granuloma.
