Localizaton of Pseudomonas aeruginosa in the Lung Tissue in Patients with Diffuse Panbronchiolitis Using an Immunofluorescent Antibody Technique and Immunoperoxydase Staining Procedure

Abstract

The purpose of this study was to identify in situ localization of P. aeruginosa in the lung of DPB patients by immunofluorescent antibody technique and immunoperoxydase staining procedures using the avidin biotinylated horseradish peroxydase complex (ABC) method.
The lung tissues were obtained by autopsy from three DPB patients who had all been in an advanced stage after a long clinical history.
P. aeruginosa was found extensively not only in the lumen of the airway from segmental bronchus to alveoli, but also in layers of ciliated epithelium, subepithelial tissue, bronchial muscle and cartilage, and in bronchial glands and the lumen of the pulmonary artery. Local response of inflammatory cells to proliferation of P. aeruginosa was minimal in these respective area.
The clinical evidence that the control of P. aeruginosa infection is especially difficult in these patients, is at least partially explainable by the results obtained in the present study.