Abstract
At gas chromatographic analysis of gases in pulmonary physiology, either the peak height or peak area is measured for quantitative analysis. Peak height is easier and more accurate to measure than the peak area. Peak area on the record should depend on the amount of the gas introduced and calibration curve for peak area should be linear, however that for peak height is usually not linear.
In this work, effect of sample volume to linearity of calibration curve was studied to reveal that calibration curve showed good linearity using sample volumes of 0.05ml for analysis of O2, N2, and Ne, 10ml for CO and 1.05ml for CO2. Reproducibility of peak height and range of error in quantitative analysis of these gases when these amounts of volume are introduced, were sufficient for practical use.
