Abstract
Rats were cauterized with moxa on the hips, and the subcutaneous and intramuscular tempetratures were kept at 45°C and at 40°C for 15min, respectively. Rats were killed by perfusion of sucrose solution under deep anesthesia and the muscle tissues of the hips were collected from the bodies at 0h, 3h and 24h after the moxibustion. The proteins were extracted from the homogenized and centrifuged tissues. Two dimensional gel electrophoreses of the proteins were carried out. Heat-shock proteins of molecular mass 70, 000 (hsp 70) and 71, 000 (hsp 71) were detected for the rat killed 3h after the moxibustion. However, no hsp 70 but hsp 71 was detected in the rats killed 0h and 24h after the moxibustion. Hsp 71 was detected in the control rat muscle as well.
