Analysis of Two-dimensional Electrophoresis Gel Pattern of Stress Proteins induced by Moxibustion and Electroacupuncture Stimulation by Image Analyze System

Abstract

Urethan-anesthetized rats were electrically stimulated for 15min by inserting acupuncture needles as electrodes into the muscle of the hips (5Hz, 3-5mA). Another rats were stimulated by moxibustion (moxa, 10mg, 10 times) for 15min. Both groups of rats were sacrificed and muscular tissues were excised 3 hours after the stimulation. The proteins extracted from the homogenized and centrifuged tissues were analyzed by two-dimensional gel electrophoreses. Stress proteins (hsp) with molecular weight of 70000 (p70), 85000 (p85) and 100000 (p100) were detected in both rats stimulated by electroacupuncture and moxibustion. Each ratio of p70, p85 and p100 to control proteins analyzed by personal image analyze system was 4.5%, 17.4% and 1.0% in electroacupuncture, 6.8%, 20.8% and 1.2% in moxibustion, respectively. The tendency of the ratios in both cases was almost similar.