Amino-acid Infusion Influences Blood Loss

Abstract

  We studied the negative effects of hypothermia on the blood coagulation system and investigated the thermogenetic effects of amino-acid infusion on the blood coagulation system using a mesenteric artery hemorrhagic model in the rat.
  Rat plasma was incubated at several different temperatures. Activated partial thromboplastin time (APTT) and prothrombin time (PT) were measured. The mesenteric artery was cut under anesthesia with or without maintaining a warm body temperature, and then bleeding time and blood loss were measured. Two-percent amino acids (800mg/kg/h×1.5h) or saline was infused into rats under anesthesia, and bleeding time and blood loss were measured.
  APTT and PT tended to be prolonged as temperature decreased. As body temperatures decreased, blood loss was significantly increased, but not bleeding time. An amino-acid infusion significantly suppressed hypothermia and decreased blood loss as compared with saline.
  The amino-acid infusion reduces blood loss in an animal model probably because of its thermogenesis.