Next-generation Sequencing Technologies, Educations, and Analysis Guidelines

Abstract

Next-generation sequencing (NGS) technologies has been widely used in medical and life sciences. A major application of NGS is RNA-seq that sequences RNAs (or transcriptomes) expressed in cells. This technique can be used to elucidate the action mechanisms of anesthesia at the transcriptome expression level. This includes to identify genes or transcripts related to anesthetic complication, hypoxia, hypothermia, concentration, and so on. This article describes NGS technologies, applications for transcriptome analyses, useful websites especially for educations, and guidelines for statistical analyses.