Abstract
We studied the sterility of the rubber septums on anesthetic vials and a transfusion bottle and evaluated the disinfection with alcoholsoaked cotton. Neither bacterial nor fungal cells were detected on the rubber septum of the vials by the culture method, while fungal cells were detected in 2 out of 10 vials after swabbing with alcohol-soaked cotton. Staphylococcus aureus and Candida albicans cells were inoculated in anesthetic vials and a transfusion bottle, and their growth was observed. The viable numbers of both organisms was reduced in the lidocaine vial, although increasing numbers were observed in the propofol vial and the transfusion bottle.