The Journal of the Japanese Society of Clinical Cytology
Online ISSN : 1882-7233
Print ISSN : 0387-1193
ISSN-L : 0387-1193
Brief Notes
The cotton method improves the recovery of cell clusters in cell block preparations from liquid-based cytology samples
Kentarou OHTAKEMasanao SAIOYoshikatsu KUROSHIMARyouko ASATONaoki YOSHIMI
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2013 Volume 52 Issue 3 Pages 271-272

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Abstract
We examined how to improve the cell recovery for cell block preparations using cervical liquid-based cytology (LBC) samples. Forty cases of both NILM and LSIL, respectively, were split into three groups and three different cell block preparation methods were applied such as nylon mesh, OCT compound and cotton. The total cell number (isolated cells and cells in cell clusters) and the incidence of cases with large clusters (≥50 cells) in specimens were counted. We found that the cotton method, by which clusters were significantly recovered, was better than the others. The cotton method is a useful tool for cell recovery in block preparations from LBC samples.
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© 2013 The Japanese Society of Clinical Cytology
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