The Journal of the Japanese Society of Clinical Cytology
Online ISSN : 1882-7233
Print ISSN : 0387-1193
ISSN-L : 0387-1193
Original Articles
A blood addition test to BD SurePathTM liquid based cytology
—Analysis of a preprocessing process removing a specimen unsatisfactory factor—
Ayana HORIGUCHI, Takashi UMEZAWA, Tomomi ASHIKAWA, Sachiko TSUCHIYA, Miyaka UMEMORI, Hiroyuki TAKAHASHI, Masahiro IKEGAMI, Kyosuke YAMADA, Aikou OKAMOTO, Kazunori OCHIAI
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2014 Volume 53 Issue 4 Pages 286-291

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Abstract
Objective : BD SurePath is the specimen manufacture system which standardized a preliminary preprocessing process removing mucus, blood, debris. We added blood in a BD SurePath and clarify performance of a preprocessing process.
Study Design : We collected squamous cells and we adjusted it to about 40 squamous cells numbers in 40× high-power fields per (HPF) one slide. We added blood to 100-3000μl in above vials, and we made a BD SurePath specimen, and a field counted squamous cells 10 in 40× HPF, and the specimen calculated mean.
Results : As for the squamous cells average count number, In 0μl (53.3), 100μl (48.2), 500μl (39.5), 1,000μl (39.1), 2,000μl (22.1), 3,000μl (10.8), there is preprocessing process.
Conclusions : A preprocessing process standardized on a BD SurePath collects an important cell in elimination and a diagnosis of an unsatisfactory specimen by blood and contributes to detection improvement of cervix lesion.
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© 2014 The Japanese Society of Clinical Cytology
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