Abstract
Objectives : Dark 3-dimensional tissue fragments which called hyperchromatic crowded cell groups (HCG) are frequently observed in the BD SurePath (SP) liquid-based cytology (LBC). In this study, we evaluated the frequency of HCG in HSIL specimens by BD SP system.
Study Design : Cytology specimens of 250 women categorized as HSIL were assessed. They were taken from women on routine cervical check-up, collected with Cervex-Brush, and processed by BD SP LBC system. This study was approved by the ethical committee of the Jikei University School of Medicine (22-189 [6366]).
Results : The patients’ age ranged from 22 and 68 years (average 38 years). HCG were observed in 69.3% of HSIL. The cervical biopsy was performed in 206 of 250 patients (82.4%).The histology showed benign in 10 patients (atrophy in 1, immature metaplasia in 9), CIN) 1 in 43, CIN2 in 99 cases, CIN3 in 47, Microinvasive squamous cell carcinoma in 7. The diagnostic accuracy in those of lesions more than CIN2 was 74.3%, and that of more than CIN1 was 95.1%.
Conclusions : HCG were frequently present in the cytology specimens with HSIL by BD SP system. Although Abnormal are easy to find on cytological screening, we must carefully differentiate them from atrophic squamous epithelium or immature metaplastic cells.
