2020 Volume 59 Issue 2 Pages 83-91
Objective : The cell block technique is useful for both final diagnosis and determining suitable cancer treatment. We invented and developed a novel unique cell block technique using tissue paper named “tissue/cell clots-by-tissue capture technique” (TxT technique).
Study Design : We examined 20 out of 96 samples of cavity fluid submitted for cytology, and further checked each TxT step and the quality of various wrapping materials. The effects of alcian blue stain for sodium alginate (SA) and TxT samples were also investigated. Quantification of immunostaining for samples obtained by TxT technique was performed.
Results : Handling of double-layered tissue was easier and more useful for tissue sectioning. Only minimal cell loss through fiber slits was observed. Tissue fibers did not affect the final diagnosis without unfavorable alcian blue stain or immunostaining results.
Conclusion : The cell block technique is essential for pathological diagnosis. Advanced preparation was not necessary for the TxT technique, and the procedure from cell sampling to preparation of paraffin blocks was very easy and quick. These preliminary results demonstrated that this technique could offer a major contribution to the further development of cytopathology and determination of cancer treatment.
