Investigation of immunocytochemical staining method in cell suspension state using microtube

Abstract

In this study, we developed a new immunocytochemistry method, which is performed on floating cells. After antigen-antibody reaction and colorization carried out under the floating condition in a microtube, the cells are on a slide glass. This floating immunocytochemical method allows stronger colorization than the conventional immunocytochemical method. This cell floating method could also improve the immunostaining by shortening the staining time in comparison with the conventional method.