2021 Volume 60 Issue 3 Pages 141-149
Objective : Rapid on-site cytologic evaluation (ROSE) of fine-needle aspiration cytology (FNAC) specimens and ultrasound-guided endoscopic fine-needle aspiration biopsy (EUS-FNAB) specimens was carried out not only to prevent degeneration of the obtained cells, but also to conduct quantitative and qualitative evaluation of the collected cells. As a staining method in ROSE, we examined the usefulness of simple Giemsa staining with wet fixation.
Study Design : The cellular morphology in simple Giemsa staining was compared to that in Papanicolaou re-staining after decolorization and refixation of the simple Giemsa-stained samples.
Results : We could easily observe metachromasia of the neuroendocrine granules in tumors with neuroendocrine differentiation, such as breast cancers and pancreatic tumors. The mucus, colloid and stroma in various tissues could also be well evaluated.
Conclusion : The wet fixation made it possible for us to observe the same cells by Papanicolaou re-staining. Moreover, we could obtain much information within a short screening time, and the wet fixation in the present method was considered to be safer in terms of the infection risk from the obtained materials than the dry fixation used in other staining methods. Thus, simple Giemsa staining with wet fixation appears to be very useful for ROSE.
