Abstract
Objective : To improve the quality of vaginal stump cytology, we examined the suitability of samples obtained using the Orcellex BrushRT oral cell collection device and processed by the BD SurePathTM (SP) method.
Study Design : We conducted vaginal stump cytology in 6260 cases during the 5-year 7-month period between March 2014 and September 2019. Samples were collected using the Orcellex BrushRT, and the tip of the brush was placed into an SP collection vial. The specimens were then prepared using the SP method and evaluated using the Bethesda system. This study was conducted with the approval of the Ethics Committee of Jikei Medical University [31-346 (9925)].
Results : The following results of cytology were obtained using the SP method : NILM (5909 cases : 94.4%), ASC-US (101 cases : 1.6%), ASC-H (16 cases : 0.3%), AGC (14 cases : 0.2%), LSIL (140 cases : 2.2%), HSIL (43 cases : 0.7%), malignant (31 cases : 0.5%), and unsatisfactory (6 cases : 0.1%). In all the unsatisfactory specimens, the cell count was found to be too low. Biopsy was performed in 23 cases (0.4%), and the rate of agreement between the SP method and histological examination was 60.9%.
Conclusion : The Orcellex BrushRT and SP method contribute to improvement of the specimen collection and sample volume, with a minimal number of unsatisfactory specimens. They can, therefore, be used as tools to achieve consistent quality in vaginal stump cytology.
