Abstract
Using a cell line (JOHYL-1) of human ovarian anaplastic disgerminoma, a sensitivity testing of anticancer drugs was studied. After exposure of anticancer agents, a cytomorphological study was made to obtain results as follows.
1) A phase-contrast microscopic examination revealed that (1) large and small prickle cells with swelling of the cytoplasm and the nucleus and (2) cytoplasmic granules.
2) Papanicolaou staining revealed that (1) a decline in staining, swelling, and vacuoles of the cytoplasm, and obscure cell membrane, (2) a reticular change in chromatin, swelling and vacuoles of the nucleus and (3) swollen and obscure patterns of the nucleori.
3) By the measurement of the major axis of the nucleus suggested that the number of the swollen nucleus corelated to the number of dead cells each other.
4) By analysis of nuclear DNA histogram by Impuls cytophotometer. 5 FU, MC and VCR showed a cycle specificity as reported previously. CQ rated as the foremost in the cytocidal effect showed stoppage of the cell cycle.
Therefore, we concluded that it was possible to state that nuclear diameter can be used as the parameters for the sensitivity testing of anticanceragents assess the relationship between swelling of the nucleus and death of the cell can be data of nuclear decline the as the parameters for the sensitivity testing of anticancer drugs.
