Abstract
Upon cytological diagnosis, Giemsa's, May-Griinwald Giemsa and Wright's stain come next to the Papanicolaou's stain. An original report of Giemsa's stain (1904), May-Grünwald-Giemsa stain (1908) and Wright's stain (1902) were cited.
Also the original method was compared with the recent modified one by using the same blood smears. The Giemsa's stain solution diluted with phosphate buffer stained better than the solution diluted with distilled water. The original May-Grünwald-Giemsa method had the same quality of staining as the variated one. The variated Wright's stain was better than the original.
With respect to the history of these three staining methods, the method began by the simple mixture of methylen blue and eosin then it was developed into the Romanowsky effect by adition of methylen azur. Then its stability was increased by the use of phosphate buffer.
Studies on simplification of these staining methods are under investigation though now three staining methods are used widely throughout the world.
