1987 Volume 26 Issue 1 Pages 23-28
The presence of HPV antigen on koilocytes noted on uterine cervical smears was studied according to the immunoperoxidase method, and their ultrastructure was observed by both scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
The results obtained were as follows:
1) The presence of HPV antigen in nucleus was confirmed in a few koilocytes by the peroxidase-antiperoxidase (PAP) method.
2) SEM film revealed some koilocytes with specific three-dimensional structure in the depressed center of the cell body.
3) TEM film showed an electron microscopically unformed portion without cytoplasmic organella around the nuclei of koilocytes. Further, virus particles thought to be HPV were noted in the nuclei of a few koilocytes.
