Detection of chlamydia trachomatis in biopsy of uterine cervicitis by immunohistochemical approach

Abstract

Detection of Chlamydia trachomatis (C. trachomatis) was attempted in 161 biopsy specimens histologically diagnosed as cervicitis by immunoperoxidase staning (peroxidase antiperoxidase method, PAP) or partly by direct immunofluorescent method.
1. 52 out of 161 (32.3%) cases proved to be positive by PAP method.
2. Age occurrence rates of C. trachomatis by PAP method are as follows;-30; 26.7%, 31-40; 33.3%, 41-50; 33.3%, 51-60; 35.3%, 61-; 16.7%.
3. Chlamydia infected cells are mainly in columnar area followed by in squamous metaplasia and in squamous epithelia.
4. Positive localization of C. trachomatis by PAP method was observed in apical portion of cytoplasm adjacent to nuclei, while positive round inclusion bodies were rere occurrence (5.8%).